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  <front>    <journal-meta>
      <journal-title>Journal of Yeast and Fungal Research </journal-title>
      <issn pub-type="epub">2141-2413</issn>      <publisher>
        <publisher-name>Academic Journals</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.5897/JYFR2015.0153</article-id>
      <title-group>
        <article-title><![CDATA[A new ethanol-based macrochemical test combined with a cultural character in the process of identification of the cosmopolitan wood-decayer, Ganoderma resinaceum Boud. (Basidiomycota)]]></article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author" xlink:type="simple">
        		        	<name name-style="western">
	            <surname>Dominique</surname>
            <given-names>Claude MOSSEBO</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Rose</surname>
            <given-names>T. AMBIT</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Marie-Claire</surname>
            <given-names>MACHOUART</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Germain</surname>
            <given-names>KANSCI</given-names>
	          </name>	
        	        </contrib>
      </contrib-group>
      <author-notes>
		<corresp id="cor1">* E-mail: <email xlink:type="simple">dmossebo@yahoo.fr</email></corresp>
      </author-notes>
      <pub-date pub-type="collection">
        <year>2015</year>
      </pub-date>
      <pub-date pub-type="epub">
      	<day>30</day>
        <month>04</month>
        <year>2015</year>
      </pub-date>
      <history>
      			<date date-type="received">
			<day>23</day>
			<month>02</month>
			<year>2015</year>
		</date>
						<date date-type="accepted">
			<day>27</day>
			<month>03</month>
			<year>2015</year>
		</date>
			  </history>
      <volume>6</volume>
      <issue>1</issue>
	  	  <fpage>1</fpage>
	  <lpage>10</lpage>
      <permissions>
		<license xlink:type="simple">
			<license-p>
			This is an open-access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
			</license-p>
		</license>
	  </permissions>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/JYFR/article-abstract/C71539B52204">
		This article is available from http://politicalwaffle.uk/journal/JYFR/article-abstract/C71539B52204	  </self-uri>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/JYFR/article-full-text-pdf/C71539B52204">
		The full text article is available as a PDF file from http://politicalwaffle.uk/journal/JYFR/article-full-text-pdf/C71539B52204	  </self-uri>
	  
      <abstract><![CDATA[A new macrochemical test using ethanol drops was set up and described here as a safer, quicker and more reliable substitute for the previously used match flame to reveal yellow resin on the pileus of Ganoderma resinaceum, no matter its geographical origin. Four concentrations (30, 70, 90 and 99%) of ethanol (CH3-CH2OH) were tested with distilled water as negative control, as a substitute to the old match flame test in the process of identification of this species. The positive control test was performed on 18 other species of Ganoderma including Ganoderma lucidum. All control tests were negative, ethanol concentrations ranging between 90-99% revealed a plusmn; bright and lasting yellowish resin oozing from the pileus of G. resinaceum. Observations from laboratory cultures showed that in this genus, only mycelium of G. resinaceum so far turns yellowish as earlier established in other studies on strains of the species identified at molecular (ITS-rDNA) level. Therefore, in this very wide genus where the boundaries between numerous species are still poorly circumscribed, the new positive ethanol test combined with the occurrence of yellowish zones in mycelial cultures bring more accuracy in the identification process of G. resinaceum, prior to confirmation by additional taxonomic investigations.

	 

	Key words: Ganoderma resinaceum, identification process, ethanol, pileus, mycelial culture, yellow resin.]]></abstract>
    </article-meta>
  </front>
      <body/>
    <back>
		<ref-list>
			<title>References</title>
						<ref id="ref1">
				<label>1</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Adaskaveg JE, Giltbertson R (1986). Cultural studies and genetics of sexuality of Ganoderma lucidum and G. tsugae in relation to the taxonomy of the G. lucidum complex. Mycologia 78:694-705.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref2">
				<label>2</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ambit RT (2011). Contribution to the taxonomic and ethnomycological study of Macromycetes growing in Mbengwi (North-West region) and surrounding areas in Cameroon. M.Sc thesis, Faculty of Science, University of Yaound 1, Cameroon, 87 p.]]>
				</mixed-citation>
			</ref>
						<ref id="ref3">
				<label>3</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Boudier EJL (1889). Etude descriptive dune nouvelle espce de Ganoderma de France: Ganoderma resinaceum sp.nov. Bull. Soc. Mycol. Fr. 5:72.]]>
				</mixed-citation>
			</ref>
						<ref id="ref4">
				<label>4</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Breitenbach J, Krnzlin F (1986). Champignons de Suisse. Tome 2. Champignons sans lames : Htrobasidiomyctes, Aphyllophorales, Gastromyctes. Edt. Mykologia, CH-6000 Lucerne 9. 412 p.]]>
				</mixed-citation>
			</ref>
						<ref id="ref5">
				<label>5</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Bresadola J (1890). Fungi kamerunenses. Bull. Soc. Mycol. Fr. 6: XXXII- XLIX.]]>
				</mixed-citation>
			</ref>
						<ref id="ref6">
				<label>6</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Chang ST, Buswell JA (1999). Ganoderma lucidum (Curt.; Fr.) P. Karst. (Aphyllophoromycetideae).  A mushrooming medicinal mushroom. Int. J. Med. Mushroom 1:139-146.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref7">
				<label>7</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Charbonnel J (1995). Les ractifs mycologiques. Tome 1. Les ractifs macrochimiques. Edit par lauteur. 344 p.]]>
				</mixed-citation>
			</ref>
						<ref id="ref8">
				<label>8</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Furtardo JS (1965). Relation of microstructure to the taxonomy of the Ganodermataceae (Polyporaceae) with special reference to the structure of the cover of the pilear surface. Mycologia 57: 688-711.]]>
				</mixed-citation>
			</ref>
						<ref id="ref9">
				<label>9</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Furtardo JS (1967). Some tropical species of Ganoderma (Polyporaceae) with pale context. Persoonia 4:379-389.]]>
				</mixed-citation>
			</ref>
						<ref id="ref10">
				<label>10</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Gilbertson RL, Ryvarden L (1986). North American Polypores, Vol. 1. AbortiporusLindtneria. Fungiflora, Oslo, Norway. 433 pp.]]>
				</mixed-citation>
			</ref>
						<ref id="ref11">
				<label>11</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Hjortstam K, Ryvarden L, Watling R (1993). Preliminary checklist of non-agaricoid macromycetes in the Korup National Park, Cameroon and surrounding area. Edinb. J. Bot. 50 (1):105-119.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref12">
				<label>12</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kengni Ayissi MB, Mossebo DC, Machouart MC, Kansci G, Tsigaing TF, Dogang LR, Metsebing BP, Djifack NM (2014b). A new method by correlation to forecast the optimal time of spore-prints production and collection on sporocarps of Ganoderma resinaceum Boud. (Basidiomycota) on natural substrate. Mycosphere 5(6): 758-767.]]>
				</mixed-citation>
			</ref>
						<ref id="ref13">
				<label>13</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kengni Ayissi MB, Mossebo DC (2014a). Some noteworthy taxonomic variations in the complex wood-decayer Ganoderma resinaceum (Basidiomycota) with reference to collections from tropical Africa. Kew Bull. 69(4):1-14.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref14">
				<label>14</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kinge TR (2012). Basal Stem Rot Disease of Oil palm and Identification of species of Ganoderma in South Western Cameroon. Ph.D Thesis, University of Buea, Cameroon. 218 p.]]>
				</mixed-citation>
			</ref>
						<ref id="ref15">
				<label>15</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kinge TR, Mih A (2011). Ganoderma ryvardense sp. nov. associated with basal stem rot (BSR) disease of oil palm in Cameroon. Mycosphere 2(2):179-188.]]>
				</mixed-citation>
			</ref>
						<ref id="ref16">
				<label>16</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kinge TR, Mih A (2014). Ganoderma lobenense (Basidiomycetes), a new species from oil palm (Elaies guineensis) in Cameroon. J. Plant Sci. 2(5): 242-245.]]>
				</mixed-citation>
			</ref>
						<ref id="ref17">
				<label>17</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kornerup A, Wansher JH (1978). Methuen handbook of colour. 3rd edn. Eyre Methuen, London. 252 p.]]>
				</mixed-citation>
			</ref>
						<ref id="ref18">
				<label>18</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Mohanty PS, Harsh NSK, Pandley A (2011). First report of Ganoderma resinaceum and G. weberianum from north India based on ITS sequence analysis and micromorphology. Mycosphere 2(4): 469-474.]]>
				</mixed-citation>
			</ref>
						<ref id="ref19">
				<label>19</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Moncalvo JM, Ryvarden L (1997). A nomenclatural Study of the Ganodermataceae Donk. Synopsis fungorum 11. 114 p.]]>
				</mixed-citation>
			</ref>
						<ref id="ref20">
				<label>20</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Moncalvo JM, Wang H, Hseu RS (1995). Phylogenetic relationships in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences. Mycologia 87(2): 223-238.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref21">
				<label>21</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Mossebo DC (2002). Growth of wood-inhabiting Lentinus species from Cameroon in laboratory culture. Mycologist 16(4):168-171.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref22">
				<label>22</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Mossebo DC (2005). Contribution  la connaissance de la flore mycologique tropicale : Inventaire, taxonomie et systmatique des collections de Basidiomyctes (Macromyctes) du Cameroun et dAfrique centrale. Mmoire dHabilitation  Diriger des Recherches (HDR), Universit de Lille 2, France. p. 1-127.]]>
				</mixed-citation>
			</ref>
						<ref id="ref23">
				<label>23</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Mossebo DC, Ryvarden L (1997). Fomitopsis africana sp. nov. (Polyporaceae, Basidiomycotina). Sydowia 49 (2): 147-149.]]>
				</mixed-citation>
			</ref>
						<ref id="ref24">
				<label>24</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Mossebo DC, Ryvarden L (2003). The Genus Mycorrhaphium in Africa. Mycotaxon 88:229-232.]]>
				</mixed-citation>
			</ref>
						<ref id="ref25">
				<label>25</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Mossebo DC, Njouonkou AL, Courtecuisse R, Amougou A (2007). Enzymatic activities and decay characteristics in some wood-rotting Basidiomycetes from Cameroon and determination of the time-dependant activity of syringaldazine in spot tests. Cryptogamie-Mycologie 28(2):107-121.]]>
				</mixed-citation>
			</ref>
						<ref id="ref26">
				<label>26</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Mossebo DC, Kengni Ayissi MB, Ambit RT (2014). New taxa and potential pharmacological properties of some Ganodermataceae (Basidiomycota) from Cameroon and central Africa. In: abstracts book (Scripta Botanica Belgica 52, 291) of the Proceedings of the XXth AETFAT Congress, Stellenbosch, South Africa, 13th-17th January 2014.]]>
				</mixed-citation>
			</ref>
						<ref id="ref27">
				<label>27</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Nnez M, Ryvarden L (2000). East Asian Polypores. Volume 1. Ganodermataceae and Hymenochataceae. Synopsis Fungorum 13. Fungiflora, Oslo, Norway. 168 p.]]>
				</mixed-citation>
			</ref>
						<ref id="ref28">
				<label>28</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Roberts P, Ryvarden L (2006). Poroid fungi from Korup National Park, Cameroon. Kew Bull. 61: 55-78.]]>
				</mixed-citation>
			</ref>
						<ref id="ref29">
				<label>29</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ryvarden L (1992). Genera of Polypores; nomenclature and taxonomy. Synopsis Fungorum 5. 292 p.]]>
				</mixed-citation>
			</ref>
						<ref id="ref30">
				<label>30</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ryvarden L (2000). Studies in neotropical polypores 2: A preliminary key to neotropical species of Ganoderma with a laccate pileus. Mycologia 92(1):180-191.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref31">
				<label>31</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ryvarden L (2004). Neotropical polypores, Part 1. Introduction, Ganodermataceae  Hymenochaetaceae. Synopsis Fungorum 19. 227 p.]]>
				</mixed-citation>
			</ref>
						<ref id="ref32">
				<label>32</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ryvarden L, Melo I (2014). Poroid fungi of Europe. Synopsis Fungorum 31. 455 p. Fungiflora, Oslo, Norway.]]>
				</mixed-citation>
			</ref>
						<ref id="ref33">
				<label>33</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ryvarden L, Johansen I, (1980). A Preliminary Polypore Flora of East Africa. Oslo, Fungiflora. 636 p.]]>
				</mixed-citation>
			</ref>
						<ref id="ref34">
				<label>34</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Steyaert RL (1967). Les Ganoderma palmicoles. Bull. J. Bot. Nat. Belg. 37 (4):465-492.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref35">
				<label>35</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Steyaert RL (1972). Species of Ganoderma and related Genera, mainly of the Bogor and Leiden Herbaria. Persoonia 7: 55-118.]]>
				</mixed-citation>
			</ref>
						<ref id="ref36">
				<label>36</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Steyaert RL (1980). Study of some Ganoderma species. Bull. J. Bot. Nat. Belg. 50:135-186.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref37">
				<label>37</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Zoberi MH (1972). Tropical macrofungi. Some common species. The MacMillan Press Ltd, London and Basingstoke.]]>
				</mixed-citation>
			</ref>
					</ref-list>
	</back>
    </article>