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<article xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" article-type="research-article" dtd-version="2.0" xml:lang="EN">
  <front>    <journal-meta>
      <journal-title>Journal of Veterinary Medicine and Animal Health</journal-title>
      <issn pub-type="epub">2141-2529</issn>      <publisher>
        <publisher-name>Academic Journals</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.5897/JVMAH2017.0546</article-id>
      <title-group>
        <article-title><![CDATA[Semen characteristics and fertility assessment of Sennar jackass (Equus asinus) in Ethiopia]]></article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author" xlink:type="simple">
        		        	<name name-style="western">
	            <surname>Alemayehu</surname>
            <given-names>Lemma</given-names>
	          </name>	
        	        </contrib>
      </contrib-group>
      <author-notes>
		<corresp id="cor1">* E-mail: <email xlink:type="simple">alemma2008@gmail.com</email></corresp>
      </author-notes>
      <pub-date pub-type="collection">
        <year>2017</year>
      </pub-date>
      <pub-date pub-type="epub">
      	<day>31</day>
        <month>07</month>
        <year>2017</year>
      </pub-date>
      <history>
      			<date date-type="received">
			<day>02</day>
			<month>01</month>
			<year>2017</year>
		</date>
						<date date-type="accepted">
			<day>27</day>
			<month>04</month>
			<year>2017</year>
		</date>
			  </history>
      <volume>9</volume>
      <issue>7</issue>
	  	  <fpage>149</fpage>
	  <lpage>153</lpage>
      <permissions>
		<license xlink:type="simple">
			<license-p>
			This is an open-access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
			</license-p>
		</license>
	  </permissions>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/JVMAH/article-abstract/54ED4A764798">
		This article is available from http://politicalwaffle.uk/journal/JVMAH/article-abstract/54ED4A764798	  </self-uri>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/JVMAH/article-full-text-pdf/54ED4A764798">
		The full text article is available as a PDF file from http://politicalwaffle.uk/journal/JVMAH/article-full-text-pdf/54ED4A764798	  </self-uri>
	  
      <abstract><![CDATA[The aim of this study was to evaluate the semen characteristics and fertility of Sennar jacks. Semen was collected between January and March using Missouri AV model and was subjected to gross and microscopic evaluation. Fertility was evaluated from pregnancy rate after AI was carried out with fresh semen on 12 Abyssinian jennies, 5 Sennar jennies and 17 local mares. Mean (plusmn;SD) total and gel-free volume; and spermatozoa concentration were 61.1plusmn;12.6 ml, 50.3plusmn;12.3 ml, and 257plusmn; 8.1 x 106/ml, respectively. Total and progressive sperm motility, sperm viability and abnormal sperm percentage were 84.2plusmn;2.1; 67.4 plusmn; 6.1%, 89%, and 10.9plusmn;2.9, respectively. There was no significant individual difference in most semen parameters. Pregnancy rate was 40% (2/5) in Sennar jennie, 58.3% (7/12) in Abyssinian jennies and 64.7% (11/17) in mares. The study thus revealed that semen can be successfully collected and evaluated as part of a breeding soundness examination of Sennar jacks during cross breeding using AI. Further study on cryopreservation of semen and improving pregnancy rate after extending semen in optimized donkey semen extender is necessary in the future.

	 

	Key words: AI, fertility, Sennar jacks, semen, Ethiopia.]]></abstract>
    </article-meta>
  </front>
      <body/>
    <back>
		<ref-list>
			<title>References</title>
						<ref id="ref1">
				<label>1</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Alemu GW, Azage T, Alemu Y (1997). Research needs of donkey utilization in Ethiopia. Improving Donkey Utilization in Ethiopia. In: Proceeding of Animal Traction Network for Eastern and Southern Africa (ATNESA) Workshop held in Debre Zeit, Ethiopia, 5-9th May. pp. 77-81.]]>
				</mixed-citation>
			</ref>
						<ref id="ref2">
				<label>2</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Allen WR, Tiplady C, Wilsher S, Lefranc AC, Morris LHA(2001). Videoendoscopic Low-Dose Uterotubal Insemination in the Mare; Proceedings of the Second Meeting of the, European Equine Gamete Group, Havemeyer Found. Monogr. Ser. 5:29.]]>
				</mixed-citation>
			</ref>
						<ref id="ref3">
				<label>3</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Arav A, Saragusty J (2013). Directional freezing of spermatozoa and embryos. Reprod. Fertil. Dev. 26:83-90.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref4">
				<label>4</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Canisso IF, Carvalho GR, Morel MD, Ker PG, Rodrigues AL, Silva EC, Coutinho Da Silva MA (2011). Seminal parameters and field fertility of cryopreserved donkey jack semen after insemination of horse mares. Equine Vet. J. 43:179-183.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref5">
				<label>5</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Central Statistical Agency (CSA), (2011). Agricultural sample census, livestock sample census year 2010 by Central statistics agency, Addis Ababa, Ethiopia.]]>
				</mixed-citation>
			</ref>
						<ref id="ref6">
				<label>6</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Contri A, Gloria A, Robbe D, Sfirro MP, Carluccio A (2012). Effect of sperm concentration on characteristics of frozen-thawed semen in donkeys. Anim. Reprod. Sci. 136:74-80.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref7">
				<label>7</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Davies Morel MCG (1999). Equine Artificial Insemination. CABI Publishing, Wallingford, UK. P 416.]]>
				</mixed-citation>
			</ref>
						<ref id="ref8">
				<label>8</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Fesseha G (1991). Use of equine in Ethiopia. Proceedings of Fourth Livestock Improvement Conference. Institute of Agricultural Research, 13-15 November, Addis Ababa, Ethiopia. 1:51-58.]]>
				</mixed-citation>
			</ref>
						<ref id="ref9">
				<label>9</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Hagstrom DJMS (2004). Donkeys are Different; An Overview of Reproductive Variations from Horses, Equine Extension, University of Illinois. pp. 4-5.]]>
				</mixed-citation>
			</ref>
						<ref id="ref10">
				<label>10</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Henry M, McDonnell SM, Lodi LD, Gastal EL (1991). Pasture mating behavior of donkeys (Equus asinus) at natural and induced estrus. J. Reprod. Fertil. 44:77-86.]]>
				</mixed-citation>
			</ref>
						<ref id="ref11">
				<label>11</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Lemma A, Derresa B (2009).Study on Reproductive Activity and Evaluation of Breeding Soundness of Jacks (Equus asinus) in and Around Debre Zeit, Ethiopia. Age 8(1.21):1-21.]]>
				</mixed-citation>
			</ref>
						<ref id="ref12">
				<label>12</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Okazaki T, Akiyoshi T, Kan M, Mori M, Teshima H, Shimada M (2012). Artificial insemination with seminal plasma improves the reproductive performance of frozen-thawed boar epididymal spermatozoa. J. Androl. 33: 990-998.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref13">
				<label>13</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Oliveira JV, Alvarenga MA, Melo CM, Macedo LM, DellAqua JA, Papa Jr. FO (2006). Effect of cryoprotectant on donkey semen freezability and fertility. Anim. Reprod. 94:82-84.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref14">
				<label>14</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Pearson RA, Quassat M (2000). A guide to live weight estimation and body condition scoring of donkeys. UK, J. Thomson Colors Printers Ltd. P 21.]]>
				</mixed-citation>
			</ref>
						<ref id="ref15">
				<label>15</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Purdy SR (2005). Artificial Insemination for Miniature Donkeys. In: Veterinary Care of Donkeys, Matthews N.S. and Taylor T.S. (Eds.). International Veterinary Information Service, Ithaca NY. Available at:
				
					View]]>
				</mixed-citation>
			</ref>
						<ref id="ref16">
				<label>16</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Qeusada F, Dorado J, Acha D, Ortiz I, Urbano M, Ramirez L, Galvez MJ, Alcaraz L, Portero JM, Gonzalez C, Demyda-Peyras S (2012). Freezing of donkey semen after 24 hours of cool storage: Preliminary results. Reprod. Fertil. Dev. 25:154.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref17">
				<label>17</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Rota A, Magelli C, Panzani D, Camillo F (2008). Effect of extender, centrifugation and removal of seminal plasma on cooled-preserved Amiata donkey spermatozoa. Theriogenology 69:176-185.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref18">
				<label>18</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Rota A, Panzani D, Sabatini C, Camillo F (2012). Donkey jack (Equus asinus) semen cryopreservation: studies of seminal parameters, post breeding inflammatory response, and fertility in donkey jennies. Theriogenology 78:1846-1854.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref19">
				<label>19</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Sabatini C, Mari G, Mislei B, Love CC, Panzani D, Camill F, Rota A (2014). Effect of post-thaw addition of seminal plasma on motility, viability and chromatin integrity of cryopreserved donkey jack (Equus asinus) spermatozoa. Reprod. Domest. Anim. 49:989-994.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref20">
				<label>20</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Samper JC (2005). Stallion semen cryopreservation: male factors affecting pregnancy rates. Proceedings of the Society for Theriogenology. San Antonio Texas. pp.160-165.]]>
				</mixed-citation>
			</ref>
						<ref id="ref21">
				<label>21</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Saragusty J (2015). Directional freezing for large volume cryopreservation. In: Wolkers WF, Oldenhof H, editors. Methods in Cryopreservation and Freeze-Drying. New York: Springer Verlarg. pp. 381-397.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref22">
				<label>22</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Squires EL, Barbacini S, Necchi D, Reger HP, Bruemmer JE (2003). Simplified Strategy for Insemination of Mares with Frozen Semen, 49th Annual Convention of the American Association of Equine Practitioners, 2003-New Orleans, LA, USA, (Ed.). Publisher: American Association of Equine Practitioners, Lexington KY. Internet Publisher: International Veterinary Information Service, Ithaca NY (www.ivis.org), 21-Nov; P0654.1103.]]>
				</mixed-citation>
			</ref>
						<ref id="ref23">
				<label>23</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Vidament M, Vincent P, Martin FX, Magistrini M, Blesbois E (2009). Differences in ability of jennies and mares to conceive with cooled and frozen semen containing glycerol or not. J. Anim. Reprod. Sci. 112:22-35.
					]]>
				</mixed-citation>
			</ref>
					</ref-list>
	</back>
    </article>