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  <front>    <journal-meta>
      <journal-title>Journal of Developmental Biology and Tissue Engineering</journal-title>
      <issn pub-type="epub">2141-2251</issn>      <publisher>
        <publisher-name>Academic Journals</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.5897/JDBTE.9000007</article-id>
      <title-group>
        <article-title><![CDATA[Gastrimargus musicus muscle tissue culture]]></article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author" xlink:type="simple">
        	          <name name-style="western">
            <surname>Shukla</surname>
            <given-names>P.</given-names>
          </name>
                    <name name-style="western">
            <surname>Singh</surname>
            <given-names>A.</given-names>
          </name>
                    <name name-style="western">
            <surname>Gawri</surname>
            <given-names>S.</given-names>
          </name>
                  </contrib>
      </contrib-group>
      <author-notes>
		<corresp id="cor1">* E-mail: <email xlink:type="simple">prashant19782000@gmail.com</email></corresp>
      </author-notes>
      <pub-date pub-type="collection">
        <year>2011</year>
      </pub-date>
      <pub-date pub-type="epub">
      	<day>30</day>
        <month>01</month>
        <year>2011</year>
      </pub-date>
      <history>
      					<date date-type="accepted">
			<day>24</day>
			<month>11</month>
			<year>2010</year>
		</date>
			  </history>
      <volume>3</volume>
      <issue>1</issue>
	  	  <fpage>11</fpage>
	  <lpage>12</lpage>
      <permissions>
		<license xlink:type="simple">
			<license-p>
			This is an open-access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
			</license-p>
		</license>
	  </permissions>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/JDBTE/article-abstract/EDC3A1E10328">
		This article is available from http://politicalwaffle.uk/journal/JDBTE/article-abstract/EDC3A1E10328	  </self-uri>
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		The full text article is available as a PDF file from http://politicalwaffle.uk/journal/JDBTE/article-full-text-pdf/EDC3A1E10328	  </self-uri>
	  
      <abstract><![CDATA[Cells from legs of Gastrimargus musicus, a grey brown grasshopper were grown in simple media. The medium was tested for growth of muscle cells and with supplements. It was found that the medium containing calcium carbonate had round cells growing close to each other in clumps. The medium containing creatine had cells growing which were elongated and branched and had all the features of fully developed muscle tissue. The muscle tissues grew to a size, more than double that of the original grasshopper and were maintained in the medium supplemented with creatine for more than two months.   

	Key words:  Gastrimargus musicus, cell culture medium, insect cell culture, muscle]]></abstract>
    </article-meta>
  </front>
  </article>