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  PUBLIC "-//NLM//DTD Journal Publishing DTD v2.0 20040830//EN" "http://dtd.nlm.nih.gov/publishing/2.0/journalpublishing.dtd">
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  <front>    <journal-meta>
      <journal-title>Journal of Clinical Medicine and Research</journal-title>
      <issn pub-type="epub">2141-2235</issn>      <publisher>
        <publisher-name>Academic Journals</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.5897/JCMR11.028</article-id>
      <title-group>
        <article-title><![CDATA[Distribution of SPV genes, plasmid profiles and pulsotypes of Salmonella enteritidis isolates of animal and human origins in selected locations of Zimbabwe]]></article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author" xlink:type="simple">
        		        	<name name-style="western">
	            <surname>Agness</surname>
            <given-names>Farai Nhidza</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Bamusi</surname>
            <given-names>Saidi</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Pious</surname>
            <given-names>Vengesai Makaya</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Pride</surname>
            <given-names>Kanyoka</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Idah</surname>
            <given-names>Sithole-Niang</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Dexter</surname>
            <given-names>Savadye</given-names>
	          </name>	
        	        </contrib>
      </contrib-group>
      <author-notes>
		<corresp id="cor1">* E-mail: <email xlink:type="simple">nhidzaagness@gmail.com</email></corresp>
      </author-notes>
      <pub-date pub-type="collection">
        <year>2012</year>
      </pub-date>
      <pub-date pub-type="epub">
      	<day>31</day>
        <month>05</month>
        <year>2012</year>
      </pub-date>
      <history>
      					<date date-type="accepted">
			<day>27</day>
			<month>04</month>
			<year>2012</year>
		</date>
			  </history>
      <volume>4</volume>
      <issue>5</issue>
	  	  <fpage>63</fpage>
	  <lpage>74</lpage>
      <permissions>
		<license xlink:type="simple">
			<license-p>
			This is an open-access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
			</license-p>
		</license>
	  </permissions>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/JCMR/article-abstract/62103934907">
		This article is available from http://politicalwaffle.uk/journal/JCMR/article-abstract/62103934907	  </self-uri>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/JCMR/article-full-text-pdf/62103934907">
		The full text article is available as a PDF file from http://politicalwaffle.uk/journal/JCMR/article-full-text-pdf/62103934907	  </self-uri>
	  
      <abstract><![CDATA[A total of 49 Salmonella enteritidis isolates identified by culture and biochemical tests were confirmed using multiplex polymerase chain reaction (PCR). The isolates were grouped into two, those with Salmonella plasmid virulence (spv) gene and those without the spv gene. Plasmid and pulsed field gel electrophoresis (PFGE) analysis were performed on both groups. Of the 49 S. enteritidis isolates identified, 39 possessed a spvgene. Moreover, of the 39 isolates possessing the spv genes, 32 were from animals and 7 from humans. Those isolates without the spv genes comprised of 5 isolates from humans and 5 from animals. Plasmid analysis discriminated the isolates with the spvgenes into 11 profiles and isolates without spv gene into 3 profiles. PFGE discriminatedS. enteritidis isolates into 10 pulsotypes (9 from animals and 1 from the human outbreak strains). There was no correlation between the pulsotypes or plasmid profiles with the geographical location of isolate. However, there was a link between strain type and source of isolate. The study was performed to characterize S. enteritidis strains using molecular typing techniques and determine their geographical distribution in Zimbabwe. The information obtained can give background information for further studies onSalmonella epidemiology in Zimbabwe.

	 

	Key words: spv, Salmonella enteritidis, pulsotypes, plasmid profiles, multiplex polymerase chain reaction (PCR), pulsed field gel electrophoresis (PFGE).]]></abstract>
    </article-meta>
  </front>
  </article>