<!DOCTYPE article
  PUBLIC "-//NLM//DTD Journal Publishing DTD v2.0 20040830//EN" "http://dtd.nlm.nih.gov/publishing/2.0/journalpublishing.dtd">
<article xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" article-type="research-article" dtd-version="2.0" xml:lang="EN">
  <front>    <journal-meta>
      <journal-title>African Journal of Plant Science</journal-title>
      <issn pub-type="epub">1996-0824</issn>      <publisher>
        <publisher-name>Academic Journals</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.5897/AJPS2025.2408</article-id>
      <title-group>
        <article-title><![CDATA[Analysis of ionome density variation in African leafy vegetables (ALVs) for nutraceutical mineral-rich (NMR) prebreeding]]></article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author" xlink:type="simple">
        		        	<name name-style="western">
	            <surname>Levi</surname>
            <given-names>Shadeya-Mudogo Akundabweni</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Hezekiah</surname>
            <given-names>Opiyo Orwa</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Solomon</surname>
            <given-names>I. Shibairo</given-names>
	          </name>	
        	        </contrib>
      </contrib-group>
      <author-notes>
		<corresp id="cor1">* E-mail: <email xlink:type="simple">lakundabweni@mmust.ac.ke</email></corresp>
      </author-notes>
      <pub-date pub-type="collection">
        <year>2025</year>
      </pub-date>
      <pub-date pub-type="epub">
      	<day>30</day>
        <month>09</month>
        <year>2025</year>
      </pub-date>
      <history>
      			<date date-type="received">
			<day>30</day>
			<month>04</month>
			<year>2025</year>
		</date>
						<date date-type="accepted">
			<day>08</day>
			<month>07</month>
			<year>2025</year>
		</date>
			  </history>
      <volume>19</volume>
      <issue>5</issue>
	  	  <fpage>104</fpage>
	  <lpage>115</lpage>
      <permissions>
		<license xlink:type="simple">
			<license-p>
			This is an open-access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
			</license-p>
		</license>
	  </permissions>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/AJPS/article-abstract/F65794873755">
		This article is available from http://politicalwaffle.uk/journal/AJPS/article-abstract/F65794873755	  </self-uri>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/AJPS/article-full-text-pdf/F65794873755">
		The full text article is available as a PDF file from http://politicalwaffle.uk/journal/AJPS/article-full-text-pdf/F65794873755	  </self-uri>
	  
      <abstract><![CDATA[African leafy vegetables (ALVs) are micronutrient-rich orphan crops with emerging potential in nutraceutical biofortification. This study analyzed 26 ALV variants across eight species cultivated in western Kenya, evaluating leaf ionome profiles for six elements (K, Ca, Fe, Sr, Mn, Zn) using X-ray fluorescence and MIR spectroscopy. Soil parameters were also profiled to assess edaphic influences. Principal Component Analysis (PCA) and K-means clustering revealed four nutrient-defined groups: Cluster 1 (Mn-Zn rich), Cluster 2 (Fe-Sr dense), Cluster 0 (balanced K-Ca), and Cluster 3 (moderate Zn-Mn). Strong correlations were observed between Ca-Sr (r = 0.879) and Mn-Zn (r = 0.515), indicating co-accumulation patterns. Cluster analysis and elemental profiling revealed Amaranth 23 and Cowpea 19 as dual-enriched accessions with particularly high iron and strontium concentrations (3810 and 143 ppm for Amaranth 23; 3200 and 154 ppm for Cowpea 19, respectively), marking them as promising candidates for Fe-targeted biofortification. These findings are based on observed Fe-Sr co-accumulation patterns rather than composite scoring. Pumpkin leaves and Kale-22 displayed broad-spectrum micronutrient profiles, suitable for addressing multiple deficiencies. The integration of ionomic profiling with multivariate statistics and soil analysis provides a robust framework for pre-breeding pipelines. These findings underscore the value of ALVs in dietary diversification, community seed exchange, and strategic biofortification, contributing to regional food and mineral micronutrient nutrition security.

	Key words: African leafy vegetables (ALVs), biofortification, ionomics, multivariate clustering, nutraceutical prebreeding, nutrient density, X-ray fluorescence (XRF) analysis.]]></abstract>
    </article-meta>
  </front>
      <body/>
    <back>
		<ref-list>
			<title>References</title>
						<ref id="ref1">
				<label>1</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Bailey RL, West KP, Black RE (2015). The epidemiology of global micronutrient deficiencies. Annals of Nutrition and Metabolism 66(suppl 2):22-33.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref2">
				<label>2</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Balarajan Y, Ramakrishnan U, zaltin E, Shankar AH, Subramanian SV (2011). Anaemia in low-income and middle-income countries. The Lancet 378(9809):2123-2135.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref3">
				<label>3</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Bnziger M, Edmeades GO, Beck D, Bellon M (2000). Breeding for drought and nitrogen stress tolerance in maize: From theory to practice, CIMMYT.]]>
				</mixed-citation>
			</ref>
						<ref id="ref4">
				<label>4</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Baxter I (2010). Ionomics: the functional genomics of elements. Briefings in Functional Genomics 9(2):149-156.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref5">
				<label>5</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Baxter I, Dilkes BP (2012). Elemental profiles reflect plant adaptations to the environment. Science 336(6089):1661-1663.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref6">
				<label>6</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Beyer P (2010). Golden Rice and Golden crops for human nutrition. New Biotechnology 27(5):478-81.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref7">
				<label>7</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Almira Casellas MJ, Prez?Martn L, Busoms S, Boesten R, Llugany M, Aarts MG, Poschenrieder C (2023). A genome?wide association study identifies novel players in Na and Fe homeostasis in Arabidopsis thaliana under alkaline?salinity stress. The Plant Journal 113(2):225-245.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref8">
				<label>8</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Dakora FD, Phillips DA (2002). Root exudates as mediators of mineral acquisition in low-nutrient environments. Plant and Soil 245(1):35-47.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref9">
				<label>9</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Golden MH (1995). Specific deficiencies versus growth failure: Type I and Type II nutrients. SCN News 12:10-14.]]>
				</mixed-citation>
			</ref>
						<ref id="ref10">
				<label>10</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Google Maps (2024). Google Maps [Map]. Available at:
				
					View]]>
				</mixed-citation>
			</ref>
						<ref id="ref11">
				<label>11</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Krmer U (2024). Metal homeostasis in land plants: a perpetual balancing act beyond the fulfilment of metalloproteome cofactor demands. Annual Review of Plant Biology 75:27-65.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref12">
				<label>12</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Guerinot ML (2000). The ZIP family of metal transporters. Biochimica et Biophysica Acta - Biomembranes 1465(1-2):190-198.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref13">
				<label>13</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Guerinot ML, Yi Y (1994). Iron: nutritious, noxious, and not readily available. Plant Physiology 104(3):815-820.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref14">
				<label>14</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[HarvestPlus (2025). Enriching 100 million lives: Biofortified crops for nutrition security. International Food Policy Research Institute.
				
					View]]>
				</mixed-citation>
			</ref>
						<ref id="ref15">
				<label>15</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Heffner EL, Sorrells ME, Jannink J-L (2009). Genomic selection for crop improvement. Crop Science 49(1):1-12.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref16">
				<label>16</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Hussain D, Haydon MJ, Wang Y, Wong E, Sherson SM, Young J, Camakaris J, Cobbett CS (2004). P-type ATPase heavy metal transporters with roles in essential zinc homeostasis in Arabidopsis. The Plant Cell 16(5):1327-1339.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref17">
				<label>17</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[International Food Policy Research Institute (IFPRI) (2014). Global nutrition report 2014: Actions and accountability to accelerate the worlds progress on nutrition. Washington, DC: IFPRI.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref18">
				<label>18</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Jones DL, Hinsinger P (2008). The rhizosphere: complex by design. Plant and Soil 312(1-2):1-6.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref19">
				<label>19</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kaplan B, Sherman T, Fromm H. (2007). Cyclic nucleotide gated channels in plants. FEBS Letters 581(12):2237-2246.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref20">
				<label>20</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kumssa DB, Joy EJM, Ander EL, Watts MJ, Young SD, Walker S, Broadley MR (2016). Dietary calcium and zinc deficiency risks are decreasing but remain prevalent. Scientific Reports 5:10974.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref21">
				<label>21</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Lahner B, Gong J, Mahmoudian M, Smith EL, Abid KB, Rogers EE, Guerinot ML, Harper JF, Ward JM, McIntyre L, Schroeder JI (2003). Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana. Nature Biotechnology 21(10):1215-1221.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref22">
				<label>22</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[OpenStreetMap Contributors (2024). OpenStreetMap [Map].
				
					View]]>
				</mixed-citation>
			</ref>
						<ref id="ref23">
				<label>23</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Palmgren MG (2001). Plant plasma membrane H+-ATPases: powerhouses for nutrient uptake. Annual Review of Plant Biology 52(1):817-845.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref24">
				<label>24</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ployet R, Feng K, Zhang J, Baxter I, Glasgow DC, Andrews HB, Rodriguez M, Chen JG, Tuskan GA, Tschaplinski TJ, Weston DJ (2024). Elemental profiling and genome-wide association studies reveal genomic variants modulating ionomic composition in Populus trichocarpa leaves. Frontiers in Plant Science 15:1450646.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref25">
				<label>25</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ritchie H, Reay DS, Higgins P (2018). Quantifying, projecting, and addressing Indias hidden hunger. Frontiers in Sustainable Food Systems 2:11.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref26">
				<label>26</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Salt DE, Baxter I, Lahner B (2008). Ionomics and the study of the plant ionome. Annual Review of Plant Biology 59:709-733.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref27">
				<label>27</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Sparks DL (2019). Fundamentals of soil chemistry. Encyclopedia of Water: Science, Technology and Society 2:1-11.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref28">
				<label>28</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Tadele Z (2019). Orphan crops: Their importance and the urgency of improvement. Planta 250(3):677-694.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref29">
				<label>29</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Tilman D, Clark M (2014). Global diets link environmental sustainability and human health. Nature 515(7528):518-522.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref30">
				<label>30</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Tripathi L, Ntui VO, Ron M, Muiruri SK (2021). CRISPR/Cas9: A breakthrough in agri-biotechnology for orphan crops. Frontiers in Genetics 12:707253.]]>
				</mixed-citation>
			</ref>
						<ref id="ref31">
				<label>31</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[University of Texas Libraries (n.d.). Africa and Kenya maps. Perry-Castaeda Library Map Collection.
				
					View]]>
				</mixed-citation>
			</ref>
						<ref id="ref32">
				<label>32</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[White PJ, Broadley MR (2009). Biofortification of crops with seven mineral elements often lacking in human diets-iron, zinc, copper, calcium, magnesium, selenium and iodine. New Phytologist 182(1):49-84.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref33">
				<label>33</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[World Health Organization (WHO) (2020). Micronutrient deficiencies.
				
					View]]>
				</mixed-citation>
			</ref>
						<ref id="ref34">
				<label>34</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Zhang J, Tuskan GA, Muchero W (2020). Genome-wide association studies identify candidate genes for leaf ionome variation in Populus trichocarpa. The Plant Journal 101(2):505-518.]]>
				</mixed-citation>
			</ref>
						<ref id="ref35">
				<label>35</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Zhao FJ, McGrath SP (2009). Biofortification and phytoremediation. Current Opinion in Plant Biology 12(3):373-380.
					]]>
				</mixed-citation>
			</ref>
					</ref-list>
	</back>
    </article>