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  <front>    <journal-meta>
      <journal-title>African Journal of Plant Science</journal-title>
      <issn pub-type="epub">1996-0824</issn>      <publisher>
        <publisher-name>Academic Journals</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.5897/AJPS2023.2308</article-id>
      <title-group>
        <article-title><![CDATA[Occurrence and distribution of viruses associated with papaya ringspot disease in Kenya]]></article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author" xlink:type="simple">
        		        	<name name-style="western">
	            <surname>Naomi</surname>
            <given-names>Nzilani Mumo</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Elijah</surname>
            <given-names>Miinda Ateka</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>George</surname>
            <given-names>Edward Mamati</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Fredah</surname>
            <given-names>Karambu Rimberia</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>George</surname>
            <given-names>Ochieng Asudi</given-names>
	          </name>	
        	        </contrib>
      </contrib-group>
      <author-notes>
		<corresp id="cor1">* E-mail: <email xlink:type="simple">naomi.mumo@jkuat.ac.ke</email></corresp>
      </author-notes>
      <pub-date pub-type="collection">
        <year>2023</year>
      </pub-date>
      <pub-date pub-type="epub">
      	<day>30</day>
        <month>11</month>
        <year>2023</year>
      </pub-date>
      <history>
      			<date date-type="received">
			<day>29</day>
			<month>03</month>
			<year>2023</year>
		</date>
						<date date-type="accepted">
			<day>03</day>
			<month>11</month>
			<year>2023</year>
		</date>
			  </history>
      <volume>17</volume>
      <issue>10</issue>
	  	  <fpage>100</fpage>
	  <lpage>107</lpage>
      <permissions>
		<license xlink:type="simple">
			<license-p>
			This is an open-access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
			</license-p>
		</license>
	  </permissions>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/AJPS/article-abstract/9842D4171515">
		This article is available from http://politicalwaffle.uk/journal/AJPS/article-abstract/9842D4171515	  </self-uri>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/AJPS/article-full-text-pdf/9842D4171515">
		The full text article is available as a PDF file from http://politicalwaffle.uk/journal/AJPS/article-full-text-pdf/9842D4171515	  </self-uri>
	  
      <abstract><![CDATA[Papaya ringspot disease is a serious threat to papaya production in Kenya. For effective management, it is important to determine the occurrence and distribution of the viruses associated with the disease. A survey was conducted in 2017, covering a total of 103 papaya fields in major papaya production areas in the country. To determine the disease incidence, 20 plants per field were visually inspected for symptoms associated with the disease. Disease severity was evaluated on a scale of 1 to 5, while disease prevalence was determined as the proportion of fields showing disease symptoms per county expressed as a percentage. A total of 287 leaf samples were collected from surveyed fields and tested for Moroccan watermelon mosaic virus (MWMV), cowpea mild mottle virus (CpMMV), and papaya mottle-associated virus (PaMV) using polymerase chain reaction (PCR)-based techniques. The highest (71.4%) disease incidence was recorded in Kiambu County, while the lowest was recorded in Busia County (2.8%). No symptomatic plants were observed in Siaya and Bungoma (0%) counties. Disease prevalence ranged from 0 to 100%. The highest disease severity, 4.0, was reported in Baringo County; while the lowest, 2.0, was reported in Kwale, Kilifi, and Taita Taveta counties. MWMV was the most prevalent, with 140 out of 287 samples testing positive and also widespread, having been detected in 11 out of the 22 counties surveyed. PaMV was the second most prevalent, detected in 39 out of 287 samples collected and in 9 out of 22 counties. CpMMV was the least prevalent, detected in 7 out of 287 samples and in three counties. The occurrence of both MWMV and PaMV was detected in five counties, while the occurrence of PaMV and CpMMV was detected in three counties. The presence of MWMV, PaMV and CpMMV was detected in one county. Viruses associated with papaya ringspot disease in Kenya are widespread in papaya-growing regions, with some counties reporting 100% disease prevalence. The development and implementation of control strategies for the disease in the country are of paramount importance. In the future, it is important to identify factors influencing disease spread in the country for effective management.

	 

	Key words: Incidence, viral diseases, control strategies, farmers, interventions.]]></abstract>
    </article-meta>
  </front>
      <body/>
    <back>
		<ref-list>
			<title>References</title>
						<ref id="ref1">
				<label>1</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Arocha Y, Vigheri N, Nkoy-Florent B, Bakwanamaha K, Bolomphety B, Kasongo M, Betts P, Monger WA, Harju V, Mumford RA, Jones P (2008). First report of the identification of Moroccan watermelon mosaic virus in papaya in Democratic Republic of Congo. Plant Pathology 57(2):387.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref2">
				<label>2</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Gashaw G, Alemu T, Tesfaye K (2014). Evaluation of disease incidence and severity and yield loss of finger millet varieties and mycelial growth inhibition of Pyricularia grisea isolates using biological antagonists and fungicides in vitro condition. Journal of Applied Biosciences 73:5883-5901.]]>
				</mixed-citation>
			</ref>
						<ref id="ref3">
				<label>3</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ghoshal B, Sanfaon H (2015). Symptom recovery in virus-infected plants: Revisiting the role of RNA silencing mechanisms. Virology 479:167-179.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref4">
				<label>4</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[HCDA (2021). Horticulture Validated report. Nairobi, Kenya. Nairobi, Kenya. Retrieved from:
				
					View]]>
				</mixed-citation>
			</ref>
						<ref id="ref5">
				<label>5</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ibaba JD, Laing MD, Gubba A (2016). Genome sequence analysis of two South African isolates of Moroccan watermelon mosaic virus infecting cucurbits. Virus Genes 52(6):896-899.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref6">
				<label>6</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Jeyanandarajah P, Brunt AA (1993). The Natural Occurrence, Transmission, Properties and Possible Affinities of Cowpea Mild Mottle Virus. Journal of Phytopathology 137(2):148-156.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref7">
				<label>7</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kansiime MK, Rwomushana I, Mugambi I, Makale F, Lamontagne-Godwin J, Chacha D, Kibwage P, Oluyali J, Day R (2020). Crop losses and economic impact associated with papaya mealybug (Paracoccus marginatus) infestation in Kenya. International Journal of Pest Management 0(0):1-14.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref8">
				<label>8</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kidanemariam DB, Sukal AC, Abraham AD, Njuguna JN, Stomeo F, Dale JL, James, Harding MR, James AP (2019). Molecular characterisation of a putative new polerovirus infecting pumpkin (Cucurbita pepo) in Kenya. Archives of Virology 1(0123456789):0-4.]]>
				</mixed-citation>
			</ref>
						<ref id="ref9">
				<label>9</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kumar NKK, Singh HS, Kalleshwaraswamy CM (2010). Aphid (aphididae: Homoptera) vectors of papaya ringspot virus (PRSV), bionomics, transmission efficiency and factors contributing to epidemiology. Acta Horticulturae 851:431-442.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref10">
				<label>10</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Lecoq H, Vgtale SDP, Cedex M, Dafalla G, Medani W, Desbiez C, Wipf-scheibel C (2001). Biological and Molecular Characterization of Moroccan watermelon mosaic virus and a Potyvirus Isolate from Eastern Sudan. Plant Disease 85(5):547-552.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref11">
				<label>11</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Legg JP, Thresh JM (2000). Cassava mosaic virus disease in East Africa: A dynamic disease in a changing environment. Virus Research 71(1-2):135-149.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref12">
				<label>12</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Mishra R, Kumar RG, Patil BL (2016). Current Knowledge of Viruses Infecting Papaya and Their Transgenic Management. In R. K. G. et Al. (Ed.), Plant Viruses: Evolution and Management. New Delhi 110012, India:  Springer Science+Business Media Singapore pp. 189-204.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref13">
				<label>13</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Monci F, Snchez-Campos S, Navas-Castillo J, Moriones E (2002). A natural recombinant between the geminiviruses Tomato yellow leaf curl Sardinia virus and Tomato yellow leaf curl virus exhibits a novel pathogenic phenotype and is becoming prevalent in Spanish populations. Virology 303(2):317-326.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref14">
				<label>14</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Mowlick S, Akther MS, Kundu BC, Akanda AM (2008). Masking behaviour and quantitative assessment of growth and and yield reduction of papaya due to papaya ringspot virus. Bangladesh Research Publications Journal 1(3):206-214.]]>
				</mixed-citation>
			</ref>
						<ref id="ref15">
				<label>15</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Mumo NN, Mamati GE, Ateka EM, Rimberia FK, Asudi GO, Boykin LM, Machuka EM, Njuguna JN, Pelle R, Francesca S (2020). Metagenomic Analysis of Plant Viruses Associated With Papaya Ringspot Disease in Carica papaya L. in Kenya. Frontiers in Microbiology, 11.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref16">
				<label>16</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Mumo NN, Mamati GE, Ateka EM, Rimberia FK, Asudi GO (2021). Farmers Knowledge, Perception and Management Practices af Papaya Ringspot Disease in Kenya. African Journal of Horticultural Science 18(2):31-42.]]>
				</mixed-citation>
			</ref>
						<ref id="ref17">
				<label>17</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ombwara FK, Asudi GO, Rimberia FK, Ateka EM, Wamocho LS (2014). The Distribution and Prevalence of Papaya Ring Spot Virus (PRSV) in Kenyan Papaya. Acta Horticulturae (1022):119-124.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref18">
				<label>18</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Piper JK, Handley MK, Kulakow PA (1996). Incidence and severity of viral disease symptoms on eastern gamagrass within monoculture and polycultures. Agriculture Ecosystems and Environment 59:139-147.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref19">
				<label>19</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[QGIS Development Team (2019). QGIS Geographic Information System; Open Source Geospatial Foundation Project, 2019.
				
					View]]>
				</mixed-citation>
			</ref>
						<ref id="ref20">
				<label>20</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Rimberia FK, Wamocho LS (2014). Papaya industry in Kenya: Production, consumption and outlook. Acta Horticulturae (1022):181-188.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref21">
				<label>21</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Schreinemachers P, Balasubramaniam S, Boopathi NM, Ha CV, Kenyon L, Praneetvatakul S, Sirijinda A, Le NT, Srinivasan R, Wu MH (2015). Farmers perceptions and management of plant viruses in vegetables and legumes in tropical and subtropical Asia. Crop Protection 75:115-123.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref22">
				<label>22</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Singh V, Shukla K (2011). Influence of inoculation time on severity of virus disease caused by Papaya ringspot virus. Annals of Plant Protection Sciences 19(1):142-146.]]>
				</mixed-citation>
			</ref>
						<ref id="ref23">
				<label>23</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Sseruwagi P, Sserubombwe WS, Legg JP, Ndunguru J, Thresh JM (2004). Methods of surveying the incidence and severity of cassava mosaic disease and whitefly vector populations on cassava in Africa: A review. Virus Research 100(1):129-142.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref24">
				<label>24</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Stevens WA (1983). Virology of flowering plants (1st ed.). Springer, Boston, MA.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref25">
				<label>25</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Woolhouse MEJ, Haydon DT, Antia R (2005). Emerging pathogens: The epidemiology and evolution of species jumps. Trends in Ecology and Evolution 20(5):238-244.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref26">
				<label>26</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Yakoubi S, Desbiez C, Fakhfakh H, Wipf-Scheibel C, Marrakchi M, Lecoq H (2008). Biological characterization and complete nucleotide sequence of a Tunisian isolate of Moroccan watermelon mosaic virus. Archives of Virology 153(1):117-125.
					]]>
				</mixed-citation>
			</ref>
					</ref-list>
	</back>
    </article>