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  <front>    <journal-meta>
      <journal-title>Journal of Veterinary Medicine and Animal Health</journal-title>
      <issn pub-type="epub">2141-2529</issn>      <publisher>
        <publisher-name>Academic Journals</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.5897/JVMAH2023.1055</article-id>
      <title-group>
        <article-title><![CDATA[Evaluation of the coccidiocidal effects of Vernonia amygdalina and Aloe vera aqueous extracts on poultry in Bambili, North West, Cameroon]]></article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author" xlink:type="simple">
        		        	<name name-style="western">
	            <surname>Ebanga</surname>
            <given-names>Echi Joan Eyong</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Itoatim</surname>
            <given-names>Betsy Endong</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Wini</surname>
            <given-names>Goudoungou</given-names>
	          </name>	
        	        </contrib>
      </contrib-group>
      <author-notes>
		<corresp id="cor1">* E-mail: <email xlink:type="simple">joan.ebangaechi@gmail.com</email></corresp>
      </author-notes>
      <pub-date pub-type="collection">
        <year>2024</year>
      </pub-date>
      <pub-date pub-type="epub">
      	<day>31</day>
        <month>01</month>
        <year>2024</year>
      </pub-date>
      <history>
      			<date date-type="received">
			<day>19</day>
			<month>12</month>
			<year>2023</year>
		</date>
						<date date-type="accepted">
			<day>08</day>
			<month>02</month>
			<year>2024</year>
		</date>
			  </history>
      <volume>16</volume>
      <issue>1</issue>
	  	  <fpage>12</fpage>
	  <lpage>25</lpage>
      <permissions>
		<license xlink:type="simple">
			<license-p>
			This is an open-access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
			</license-p>
		</license>
	  </permissions>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/JVMAH/article-abstract/82E609371888">
		This article is available from http://politicalwaffle.uk/journal/JVMAH/article-abstract/82E609371888	  </self-uri>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/JVMAH/article-full-text-pdf/82E609371888">
		The full text article is available as a PDF file from http://politicalwaffle.uk/journal/JVMAH/article-full-text-pdf/82E609371888	  </self-uri>
	  
      <abstract><![CDATA[Coccidiosis is a disease transmitted through ingestion of contaminated food or water with sporulated Eimeria oocysts. Bloody diarrhoea is the primary symptom. Farm poultry production systems are recently challenged with the concept of quot;clean, green, ethicalquot; which promotes limited use of drugs, chemicals and hormones for poultry welfare. Substituting synthetic drugs with plant-based supplements such as Vernonia amygdalina and Aloe vera could ensure healthier food for humans. This study aimed at evaluating the use of these plant extracts in controlling chicken coccidiosis. Chicks were infected by inoculating oocysts in their water. Four chicks were placed in 24 separate cages, infection confirmed by observing for blood in faeces, followed by stool microscopy. Plant extracts were gotten by decoction and maceration extraction methods and administered at different concentrations (100, 200, 300 and 400 mg/L) to treat coccidiosis in 66 infected chicks. Chicks were weighed to check for weight gain and feed conversion ratio and growth performance. Different periods (4-9 days) were used to observe the effect of the extracts. Blood was collected from the chicks two weeks after inoculation of oocysts to determine whether these extracts could be beneficial to some haematological parameters. Concentration of 200 mg/L was the most effective dosage followed by 400 mg/L. The extracts showed some significant effects on the growth performance and on haematological parameters (Hb and platelets). Conclusively, A. vera and V. amygdalina are not only beneficial for improvement of chicken growth but also show some effects on haematological parameters and seem to be a good natural alternative for treatment of coccidiosis in chicken.

	Key words: Aloe vera, Vernonia amygdalina, plant extract, coccidiocidal, chicken.]]></abstract>
    </article-meta>
  </front>
      <body/>
    <back>
		<ref-list>
			<title>References</title>
						<ref id="ref1">
				<label>1</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Abdul Q, Amir I, Syed SA, Muhammed SK, Ismail B (2020). Effects of some alternative plant extracts used as natural coccidiostat for pigeons. Journal of Animal Science and Products 3(1):20-31.]]>
				</mixed-citation>
			</ref>
						<ref id="ref2">
				<label>2</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Abu-Akkada SS, Award AM (2012). Isolation, propagation, identification and comparative pathogenicity of five Egyptian field strains of Eimeria tenella from broiler chickens in five different provinces in Egypt. Research in Veterinary Science 92(1):92-95.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref3">
				<label>3</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Abubakar U. Mohammed B (2019). Performance of broiler chickens fed Bitter leaf and Moringa leaf meal-based diets under single phase feeding regime. Federal University of Technology, Minna, institutional repository 60 p.]]>
				</mixed-citation>
			</ref>
						<ref id="ref4">
				<label>4</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Acimovic M, Sikora V, Brdar-Jokanovi? M, Kiprovski B, Popovi? V, Koren A, Puva?a N (2019). Dracocephalum moldovica: cultivation, chemical composition and biological activity. Journal of Agronomy, Technology and Engineering Management 2(1):153-167.]]>
				</mixed-citation>
			</ref>
						<ref id="ref5">
				<label>5</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Allen PC, Fetterer RH (2012). Recent advances in biology and immunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry. Journal of Clinical Microbiology Reviews 15:58-65.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref6">
				<label>6</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Blake D (2015). Eimeria genomics: where are we now and where are we going? Veterinary Parasitology 212: 68-74.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref7">
				<label>7</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Botsoglou NA, Florou-Paneri P, Christaki E, Fletouris DJ, Spais AB (2002). Effect of dietary oregano essential oil on performance of chickens and on iron-induced lipid oxidation of breast, thigh, and abdominal fat tissues. British Journal of Poultry Science 43:223-230.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref8">
				<label>8</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Boudreau MD Beland FA (2006). An evaluation of the biological and toxicological properties of Aloe barbadensis (Miller) A. vera. Journal of Environmental Science and Health 24(1):103-54.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref9">
				<label>9</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Erasto P, Grierson DS, Afolayan AJ (2007). Anti-oxidants constituents in V. amygdalina leaves. Journal of Pharmaceutical Biology: 45(3):195-199.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref10">
				<label>10</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Gotep JG, Tanko JT, Forcados GE, Muraina TA, Ozele N, Dognyaro BB, Oladipo OO, Makoshi MS, Akanbi OB, Kinjir H, Samuel AL, Onyiche TE, Ochigbo GO, Aladelokun OB, Ozoani HA, Vigoff VZ, Dapuliga CC Atiku AA, Okewolw PA, Shamaki D, Ahmed MS, Nduaka CI (2016). Therapeutic and safety evalustion of combined aqueous extracts of Azadirachta indica and Khaya senegalensis in chickens experimentally infected with Eimeria oocysts. Journal of Parasitology Research pp. 1-9.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref11">
				<label>11</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Hamma LL, Amaza DS, Zirahei JV, Goji ADT, Mari H, Amali F (2016). Effects of aqueous extract of Bitter leaf (V. amygdalina) on phenyl-hydrazine and induced kidney disease in albino rat. International Journal of Advanced Research 4(11):39-47.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref12">
				<label>12</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Haug A, Gjevre AG, Thebo P, Mattsson JG, Kaldhusdal M (2008). Coccidial infections in commercial broilers: epidemiological aspects and comparison of Eimeria species identification by morphometric and polymerase chain reaction techniques. Avian Pathology 37(2):161-70.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref13">
				<label>13</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ige AO, Odunsi AA, Akinlade JA, Ojedapo LO, Ameen SA, Rafiu TA (2006). Gliricidia leaf meal in Layers diet: Effect on performance nutrient digestibility and economy of production. Journal of Animal and Veterinary Advances 5(6):483-486.]]>
				</mixed-citation>
			</ref>
						<ref id="ref14">
				<label>14</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Jaime S (2006). Diagnostic value of haematology. Clinical Avian Medicine 2:588-610.]]>
				</mixed-citation>
			</ref>
						<ref id="ref15">
				<label>15</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Jaiswal V, Brar APS, Sandhu BS, and Singla LD (2023). Faecal prevalence and histopathological evaluation of coccidiosis in bovine calves. Journal of Parasitic Diseases 47(3):550-555.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref16">
				<label>16</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kostadinovi L, Dozet G, Levi? J, Pavkov S, Galonja T (2010b). Uticaj esencijalnog ulja origana na karakteristike brojlera posle eksperimentalne infekcije kokcidijama. Proceedings of 51st Symposium on Oil Production pp. 265-271.]]>
				</mixed-citation>
			</ref>
						<ref id="ref17">
				<label>17</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kostadinovi? J, Levi? J (2018). Effects of phytoadditives in poultry and pigs diseases. Journal of Agronomy, Technology and Engineering Management 1(1):1-7.]]>
				</mixed-citation>
			</ref>
						<ref id="ref18">
				<label>18</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kostadinovi? L, Levi? J, Pavkov S, Kalu?erovi? D (2010a). Effect of oregano essential oil on antioxidative system of broilers blood and liver. Proceedings of the 14 International Feed Symposium, Novi Sad, Serbia pp. 282-287.]]>
				</mixed-citation>
			</ref>
						<ref id="ref19">
				<label>19</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kostadinovic L, Popovic S, Ljubojevic PD, Cabarkapa I, Duragic O, Levic J (2019). Medicinal plants as natural alternatives to coccidal synthetic drugs in broiler chicken production. Journal of Agronomy, Technology and Engineering Management 2(5):325-334.]]>
				</mixed-citation>
			</ref>
						<ref id="ref20">
				<label>20</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kostadinovi? L, Veki? L, Galonja-Coghill T, Levi? J, Pavkov S (2011). Effect of Mentha piperita on antioxidative status, growth performances and quality of poultry meat. Proceedings of the 9th International Scientific Conference Serbia Facing the Challenges of Globalisation and Sustainable Development, Belgrade pp. 295-301.]]>
				</mixed-citation>
			</ref>
						<ref id="ref21">
				<label>21</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Levi? J, ?abarkapa I, Todorovi? G, Pavkov S, Coghill-Galonja T, Kostadinovi? L (2011). In vitro evaluation of antibacterial activity of essential oils from family Lamiaceae. Romanian Biotechnological Letters 16(2):6034-6041.]]>
				</mixed-citation>
			</ref>
						<ref id="ref22">
				<label>22</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[McDougald LR, Fitz-Coy SH (2008). Protozoal infection, In YM Saif (12th edition). Diseases in poultry.]]>
				</mixed-citation>
			</ref>
						<ref id="ref23">
				<label>23</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Mohammed AA, Zakaiyau AS (2012). Bitter leaf (V. amygdalina) as a feed additive in broiler diets. Research Journal of Animal Science 6(3):38-41.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref24">
				<label>24</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Nghonjuyi NW, Tiambo CK, Kimbi H, Mankaa CN, Juliano RS, Lisita F (2015). Efficacy of ethanoic extract of Carica papaya leaves as a substitute of sulphanomide for the control of coccidiosis in KABIR chickens in Cameroon. Journal of Animal Health and Production 3(1):21-27.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref25">
				<label>25</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Oliveira AA, Segovia J, Sousa V, Mata E, Gonalves M, Bezerra R, Junior P, Kanzaki L (2013). Antimicrobial activity of Amazonian botanical supplements. Springer Plus 2:371-377.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref26">
				<label>26</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Onu PN (2010). Impact of heat-treated manure-based diets with or without exogenous enzyme on nutrient digestibility and economies of production of finisher broilers. International Journal of Science and Nature 1(1):17-21.]]>
				</mixed-citation>
			</ref>
						<ref id="ref27">
				<label>27</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Pangasa A, Singla LD, Ashuma A (2007a). Biochemical alterations in chicken during Eimeria tenella infection medicated with coccidiostats and immunomodulator. The Indian Journal for Field Veterinarians 3(2):06-10.]]>
				</mixed-citation>
			</ref>
						<ref id="ref28">
				<label>28</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Pangasa A, Singla LD, Sood N, Singh A and Juyal PD (2007b). Histopathological evaluation of anticoccidial activity of an ayurvedic coccidiostat, in induced Eimeria tenella infection in chicken. Indian Journal of Animal Sciences 77(3):214-216.]]>
				</mixed-citation>
			</ref>
						<ref id="ref29">
				<label>29</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Puva?a N (2008). Effect of phytoadditive (Allium sativum L.) in fattening chicks nutrition. Proceedings of the 32nd International Conference of Agriculture, Novi Sad pp. 116-121.]]>
				</mixed-citation>
			</ref>
						<ref id="ref30">
				<label>30</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Puva?a N, Kostadinovi? LJ, Popovi? S, Levi? J, Ljubojevi? D, Tufarelli V, Jovanovi? R, Tasi? T, Ikoni? P, Luka? D (2016). Proximate composition, cholesterol concentration and lipid oxidation of meat from chickens fed dietary spice addition (Allium sativum, Piper nigrum, Capsicum annuum). Animal Production Science 56(11):1920-1927.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref31">
				<label>31</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Puva?a N, Ljubojevi? PD, ?abarkapa I, Popovi? S, Tomi?i? Z, Nikolova N, Levi? J (2019). Quality of broiler chickens carcass fed dietary addition of garlic, black pepper and hot red pepper. Journal of Agronomy, Technology and Engineering Management 2(1):218-227.]]>
				</mixed-citation>
			</ref>
						<ref id="ref32">
				<label>32</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Sani AA, Alemika ET, Abdulraheem, RO, Abdulkareem SS, Abdulraheem RB, Ilyas M (2012). A study review of documented phytochemistry of V. amygdalina (Family Asteraceae) as the basis for pharmacologic activity of plant extract. Journal of Natural Sciences Research 2(7):1-9.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref33">
				<label>33</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Singla M, Saini AL, Singla LD, Kaswan S, Singh D, Grewal RS (2018). Effect of zinc supplementation as an alternative management intervention on coccideal load of Beetal bucks under stall-fed conditions. Indian Journal of Animal Research 52(2):314-318.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref34">
				<label>34</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Srinivasan K (2004). Spices as influencers of body metabolism: an overview of three decades of research. Food Research International Journal 38(1):77-86.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref35">
				<label>35</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Stana?ev V, Glamo?i? D, Miloevi? N, Puva?a N, Stana?ev V, Plava N (2011). Effect of garlic (Allium sativum L.) in fattening chicks nutrition. African Journal of Agricultural Research 6(4):943-948.]]>
				</mixed-citation>
			</ref>
						<ref id="ref36">
				<label>36</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Stana?ev V, Miloevi? N, Plava N, Bjedov S, Stana?ev V, Puva?a N, Arapovi? Ž (2010). Phyto additives (Allium sativum L.) in the diet of fattening chickens. Proceedings of the 14th International Symposium of Feed Technology, Novi Sad pp. 295-302.]]>
				</mixed-citation>
			</ref>
						<ref id="ref37">
				<label>37</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[UNIDO, ICS (2008). Extraction technologies for medicinal and aromatic plants. United Nations Industrial Development Organization, International Center for Science and High Technology pp. 83-95.]]>
				</mixed-citation>
			</ref>
						<ref id="ref38">
				<label>38</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Unigwe CR, Okafor UP, Ogbu UM, Nwuffor OC (2014). The nutritive profile of sun-dried paw paw (Carica papaya) leaf meal and its effect on the growth performance of boiler chickens. International Journal of Pure and Applied Science Technology 20(2):72-78.]]>
				</mixed-citation>
			</ref>
						<ref id="ref39">
				<label>39</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Yang WC, Tien YJ, Liang CL, Chang CLT (2015). Effects of Bidens Pilosa on infection and drug resistance of Eimeria in chickens. Journal of Research in Veterinary Science 98:74-81.
					]]>
				</mixed-citation>
			</ref>
					</ref-list>
	</back>
    </article>