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  <front>    <journal-meta>
      <journal-title>Journal of Microbiology and Antimicrobials</journal-title>
      <issn pub-type="epub">2141-2308</issn>      <publisher>
        <publisher-name>Academic Journals</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.5897/JMA2015.0360</article-id>
      <title-group>
        <article-title><![CDATA[Antimicrobial compounds produced by Enterococcus spp. isolates from fecal samples of wild South American fur seals]]></article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author" xlink:type="simple">
        		        	<name name-style="western">
	            <surname>Carolina</surname>
            <given-names>Baldisserotto Comerlato</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Jlia</surname>
            <given-names>Roberta Buboltz</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Naiara</surname>
            <given-names>Aguiar Santestevan</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Amanda</surname>
            <given-names>de Souza da Motta</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Ana</surname>
            <given-names>Paula Guedes Frazzon</given-names>
	          </name>	
        	        </contrib>
      </contrib-group>
      <author-notes>
		<corresp id="cor1">* E-mail: <email xlink:type="simple">ana.frazzon@ufrgs.br</email></corresp>
      </author-notes>
      <pub-date pub-type="collection">
        <year>2016</year>
      </pub-date>
      <pub-date pub-type="epub">
      	<day>30</day>
        <month>04</month>
        <year>2016</year>
      </pub-date>
      <history>
      			<date date-type="received">
			<day>31</day>
			<month>12</month>
			<year>2015</year>
		</date>
						<date date-type="accepted">
			<day>14</day>
			<month>04</month>
			<year>2016</year>
		</date>
			  </history>
      <volume>8</volume>
      <issue>3</issue>
	  	  <fpage>14</fpage>
	  <lpage>21</lpage>
      <permissions>
		<license xlink:type="simple">
			<license-p>
			This is an open-access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
			</license-p>
		</license>
	  </permissions>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/JMA/article-abstract/2CBA28D58373">
		This article is available from http://politicalwaffle.uk/journal/JMA/article-abstract/2CBA28D58373	  </self-uri>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/JMA/article-full-text-pdf/2CBA28D58373">
		The full text article is available as a PDF file from http://politicalwaffle.uk/journal/JMA/article-full-text-pdf/2CBA28D58373	  </self-uri>
	  
      <abstract><![CDATA[The aims of this study were to identify bacteriocinogenic activity in 13 enterococci isolated from fecal samples of wild South American (Arctocephalus australis) and Subantarctic fur seals (Arctocephalus tropicalis); to determine the physicochemical characteristics and antimicrobial spectrum of antimicrobial compounds against Gram-positive and Gram-negative bacteria; and to evaluate the presence of bacteriocin structural genes by PCR. Out of 13 enterococci screened for antimicrobial activity, five enterococci showed activity against Listeria monocytogenes ATCC 35152, an important pathogen linked to food. Of these, only the E. mundtii strain J5 maintained the activity after the pH was adjusted (pH 6.5). The activity of antimicrobial compounds from the E. mundtii strain J5 (ACs-J5) was lost after proteolytic enzyme treatment; however, the activity was maintained after heat, pH (acidic and basic conditions) and chemical treatment. ACs-J5 showed narrow spectrum activity. Only the mundticin KS gene was detected in the J5 strain and no plasmid was present. In conclusion, the properties presented by ACs-J5 make it a valuable biopreservative in food industries in avoiding pathogenic microorganisms such as L. monocytogenes and it should be a good candidate for probiotic application.

	Key words: Antimicrobial compounds, Enterococcus mundtii, wild fur seal, antilisterial activity.]]></abstract>
    </article-meta>
  </front>
      <body/>
    <back>
		<ref-list>
			<title>References</title>
						<ref id="ref1">
				<label>1</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Adeniyi BA, Adetoye A, Ayeni FA (2015). Antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens. Afr. Health Sci. 15(3):888-895.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref2">
				<label>2</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Almeida T, Brando A, Mu-oz-Atienza E, Gonalves A, Torres C, Igrejas G, Hernndez PE, Herranz C, Cintas LM, Poeta P (2011). Identification of bacteriocin genes in enterococci isolated from game animals and saltwater fish. J. Food Prot. 74(8):1252-1260.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref3">
				<label>3</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Bennik MH, Vanloo B, Brasseur R, Gorris LG, Smid RJ (1998). A novel bacteriocina with a YGNGV motif from vegetable-associated Enterococcus mundtii: Full characterization and interaction with target organisms. Biochim. Biophys. Acta. 1373(1):47-58.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref4">
				<label>4</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Bigwood T, Hudson JA, Cooney J, McIntyre L, Billington C, Heinemann JA, Wall F (2012). Inhibition of Listeria monocytogenes by Enterococcus mundtii isolated from soil. Food Microbiol. 32(2):354-360.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref5">
				<label>5</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Birri DJ, Brede DA, Forberg T, Holo H, Nes IF (2010). Molecular and genetic characterization of a novel bacteriocin locus in Enterococcus avium isolates from infants. Appl. Environ. Microbiol. 76(2):483-492.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref6">
				<label>6</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Brando A, Almeida T, Mu-oz-Atienza E, Torres C, Igrejas G, Hernndez PE, Cintas LM, Poeta P, Herranz C (2010). Antimicrobial activity and occurrence of bacteriocin structural genes in Enterococcus spp. of human and animal origin isolated in Portugal. Arch. Microbiol. 192(11):927-936.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref7">
				<label>7</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Criado R, Gutirrez J, Budin-Verneuil A, Hernndez PE, Hartke A, Cintas LM (2008). Molecular analysis of the replication region of the pCIZ2 plasmid from the multiple bacteriocin producer strain Enterococcus faecium L50. Plasmid 60(3):18-189.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref8">
				<label>8</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[de la Fuente-Salcido NM, Casados-Vzquez LE, Barboza-Corona JE (2013). Bacteriocins of Bacillus thuringiensis can expand the potential of this bacterium to other areas rather than limit its use only as microbial insecticide. Can. J. Microbiol. 59(8):515-522.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref9">
				<label>9</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[De Vuyst L, Foulqui Moreno MR, Revets H (2003). Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins. Int. J. Food. Microbiol. 84(3):299-318.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref10">
				<label>10</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Donato ST (2007) Comparao de mtodos convencionais e semi-automatizados para identificao de Enterococcus spp. frente a Biologia Molecular em identificaes discrepantes. Fortaleza, Brasil, 86 p. (M. Sc. Dissertation. Faculdade de Medicina. UFC).]]>
				</mixed-citation>
			</ref>
						<ref id="ref11">
				<label>11</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Espeche MC, Jurez Toms MS, Wiese B, Bru E, Nader-Macas ME (2014). Physicochemical factors differentially affect the biomass and bacteriocin production by bovine Enterococcus mundtii CRL1656. J. Dairy. Sci. 97(2):789-797.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref12">
				<label>12</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Foulqui MMR, Callewaert R, Devreese B, Van Beeumen J, De Vuyst L (2003) Isolation and biochemical characterization of enterocin produced by enterococci from different sources. J. Appl. Microbiol. 94(2):214-229.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref13">
				<label>13</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Gaamouche S, Arakrak A, Bakkali M, Laglaoui A (2014). Antimicrobial activity of lactic acid bacteria and bacteriocins isolated from a traditional brine table olives against pathogenic bacteria. Int. J. Curr. Microbiol. App. Sci. 3(11):657-666.]]>
				</mixed-citation>
			</ref>
						<ref id="ref14">
				<label>14</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Gao Y, Li B, Li D, Zhang L (2016). Purification and characteristics of a novel bacteriocin produced by Enterococcus faecalis L11 isolated from Chinese traditional fermented cucumber. Biotechnol. Lett. 67(5):1-6.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref15">
				<label>15</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Gutierrez J, Criado R, Citti R, Martn M, Herranz C, Nes I, Cintas L, Hernndez P (2005). Cloning, production and functional expression of enterocin P, a sec-dependent bacteriocin produced by Enterococcus faecium P13, in Escherichia coli. Int. J. Food Microbiol. 103(3):239-250.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref16">
				<label>16</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Kawamoto S, Shima J, Sato R, Eguchi T, Ohmomo S, Shibato J, Horikoshi N, Takeshita K (2002). Biochemical and genetic characterization of mundticin KS, an antilisterial peptide produced by Enterococcus mundtii NFRI 7393. Appl. Environ. Microbiol. 68(8):38303840.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref17">
				<label>17</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Klaenhammer TR (1993). Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev. 12:39-85.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref18">
				<label>18</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Leroy F, De Vuyst L (2002). Bacteriocin production by E. faecium RZSC5 is cell density limited and occurs in the very early growth phase. J. Food. Microbiol. 72(1-2):155-164.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref19">
				<label>19</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Lewus CB, Montville TJ (1991). Detection of bacteriocins produced by lactic-acid bacteria. J. Microbiol. Meth. 13(2):145-150.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref20">
				<label>20</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Maldonado-Barragn A, Caballero-Guerrero B, Martn V, Ruiz-Barba JL, Miguel Rodrguez J (2016). Purification and genetic characterization of gassericin E, a novel co-culture inducible bacteriocin from Lactobacillus gasseri EV1461 isolated from the vagina of a healthy woman. BCM Microbiol. 16:37.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref21">
				<label>21</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Mello JF (2007). Caracterizao molecular do gene iap de Listeria monocytogenes isoladas de alimentos no estado do Rio Grande do Sul. Rio Grande do Sul, Brasil, 156 p. (M. Sc. Dissertation. Instituto de Cincia e Tecnologia de Alimentos (UFRGS).]]>
				</mixed-citation>
			</ref>
						<ref id="ref22">
				<label>22</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Moshood AY, TengkuHaziyamin ATAH (2012) Optimization of temperature and pH for the growth and bacteriocin production of Enterococcus faecium B3L3. IOSR J. Pharm. 2(6):49-59.]]>
				</mixed-citation>
			</ref>
						<ref id="ref23">
				<label>23</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Moshood AY, Tengku Haziyamin ATAH, Abdul H (2015). Detection and characterization of enterocin encoding genes in Enterococcus mundtii strain C4l10 from the cecum of non-broiler chicken. Int. J. Biol. Pharm. Allied. Sci. 7(4):131-136.]]>
				</mixed-citation>
			</ref>
						<ref id="ref24">
				<label>24</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Motta AS, Brandelli A (2002). Characterization of an antibacterial peptide produced by Brevibacterium linens. J. Appl. Microbiol. 92(1):63-70.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref25">
				<label>25</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Motta AS, Lorenzini DM, Brandelli A (2007). Purification and partial characterization of an antimicrobial peptide produced by a novel Bacillus sp. isolated from the Amazon Basin. Curr. Microbiol. 54(4):282286.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref26">
				<label>26</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Nes IF, Diep DB, Ike Y (2014). Enterococcal bacteriocins and antimicrobial proteins that contribute to niche control. In: Gilmore MS, Clewell DB, Ike Y, et al., editors. Enterococci: From Commensals to Leading Causes of Drug Resistant Infection [Internet]. Boston: Massachusetts Eye and Ear Infirmary. Available at:View]]>
				</mixed-citation>
			</ref>
						<ref id="ref27">
				<label>27</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Park S, Itoh K, Fujisawa T (2003). Characteristics and identification of enterocins produced by Enterococcus faecium JCM 5804T. J. Appl. Microbiol. 95(2):294-300.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref28">
				<label>28</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Paschoalin VMF, Bellei B, Miguel M, Mere Del Aguila EM, Silva JT (2011). Purification of a bacteriocin produced by Enterococcus faecium and its effectiveness for preservation of fresh-cut lettuce. J. Microbiol. Antimicrob. 3(5):119-125.]]>
				</mixed-citation>
			</ref>
						<ref id="ref29">
				<label>29</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Poeta P, Costa D, Rojo-Bezares B, Zarazaga M, Klibi N, Rodrigues J, Torres C (2007).Detection of antimicrobial activities and bacteriocin structural genes in faecal enterococci of wild animals. Microbiol. Res. 162(3):257-263.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref30">
				<label>30</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Poeta P, Igrejas G, Costa D, Sargo R, Rodrigues J, Torres C (2008). Virulence factors and bacteriocins in faecal enterococci of wild boars. J. Basic. Microbiol. 48(5):385-392.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref31">
				<label>31</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Saavedra L, Minahk C, de Ruiz Holgado AP, Sesma F (2004). Enhancement of the enterocin CRL35 activity by a synthetic peptide derived from the NH2-terminal sequence. Antimicrob. Agents Chemother. 48(7):2778-2781.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref32">
				<label>32</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Sambrook J, Russel DW (2001). Molecular cloning: A laboratory manual. Cold Spring Harb Protoc, New York, NY.]]>
				</mixed-citation>
			</ref>
						<ref id="ref33">
				<label>33</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Santestevan NA, de Angelis Zvoboda D, Prichula J, Pereira RI, Wachholz GR, Cardoso LA, de Moura TM, Medeiros AW, de Amorin DB,Tavares M, dAzevedo PA, Franco AC, Frazzon J, Frazzon AP (2015). Antimicrobial resistance and virulence factor gene profiles of Enterococcus spp. isolates from wild Arctocephalus australis (South American fur seal) and Arctocephalus tropicalis (Subantarctic fur seal). World J. Microbiol. Biotechnol. 31(12):1935-1946.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref34">
				<label>34</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson MA, Roy SL, Jones JL, Griffin PM (2011). Foodborne illness acquired in the United StatesMajor pathogens. Emerg. Infect. Dis. 17(1):7-15.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref35">
				<label>35</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Xiaoyuan H, Ruoyu M, Yong Z, Da T, Xiumin W, Di X, Jianzhong H, Jianhua W (2014). Biotechnical paving of recombinant enterocin A as the candidate of anti-Listeria agent. BMC Microbiol. 14:220.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref36">
				<label>36</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Yang Y, Tao WY, Liu YJ, Zhu F (2008). Inhibition of Bacillus cereus by lactic acid bacteria starter cultures in rice fermentation. Food Control. 19(2):159-161.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref37">
				<label>37</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Yoon YC, Park HJ, Lee NK, Paik HD (2005). Characterization and enhanced production of enterocin HJ35 by Enterococcus faecium HJ35 isolated from human skin. Biotechnol. Bioproc. E10:296-303.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref38">
				<label>38</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Zendo T, Eungruttanagorn N, Fujioka S, Tashiro Y, Nomura K, Sera Y, Kobayashi G, Nakayama J, Ishizaki A, Sonomoto K (2005). Identification and production of a bacteriocin from Enterococcus mundtii QU 2 isolated from soybean. J. Appl. Microbiol. 99:1181-1190.
					]]>
				</mixed-citation>
			</ref>
					</ref-list>
	</back>
    </article>