<!DOCTYPE article
  PUBLIC "-//NLM//DTD Journal Publishing DTD v2.0 20040830//EN" "http://dtd.nlm.nih.gov/publishing/2.0/journalpublishing.dtd">
<article xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" article-type="research-article" dtd-version="2.0" xml:lang="EN">
  <front>    <journal-meta>
      <journal-title>African Journal of Plant Science</journal-title>
      <issn pub-type="epub">1996-0824</issn>      <publisher>
        <publisher-name>Academic Journals</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.5897/AJPS2023.2319</article-id>
      <title-group>
        <article-title><![CDATA[Prospecting, survey and plant material collection for Hibiscus sabdariffa L. (Malvaceae) genebank establishment in Côte d'Ivoire]]></article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author" xlink:type="simple">
        		        	<name name-style="western">
	            <surname>Gnigouan</surname>
            <given-names>Kadio Guy Roland Anzara</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Chia</surname>
            <given-names>Michelle Valrie Angui</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Saraka</surname>
            <given-names>Didier Martial Yao</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Nafan</surname>
            <given-names>Diarrassouba</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Doffou</surname>
            <given-names>Selastique Akaffou</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Raoul</surname>
            <given-names>Sylvre Sie</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Bi</surname>
            <given-names>Iri Arsne Zoro</given-names>
	          </name>	
        	        </contrib>
      </contrib-group>
      <author-notes>
		<corresp id="cor1">* E-mail: <email xlink:type="simple">guyanzara@yahoo.fr</email></corresp>
      </author-notes>
      <pub-date pub-type="collection">
        <year>2023</year>
      </pub-date>
      <pub-date pub-type="epub">
      	<day>31</day>
        <month>10</month>
        <year>2023</year>
      </pub-date>
      <history>
      			<date date-type="received">
			<day>16</day>
			<month>07</month>
			<year>2023</year>
		</date>
						<date date-type="accepted">
			<day>10</day>
			<month>10</month>
			<year>2023</year>
		</date>
			  </history>
      <volume>17</volume>
      <issue>9</issue>
	  	  <fpage>76</fpage>
	  <lpage>84</lpage>
      <permissions>
		<license xlink:type="simple">
			<license-p>
			This is an open-access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
			</license-p>
		</license>
	  </permissions>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/AJPS/article-abstract/86FDF2671482">
		This article is available from http://politicalwaffle.uk/journal/AJPS/article-abstract/86FDF2671482	  </self-uri>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/AJPS/article-full-text-pdf/86FDF2671482">
		The full text article is available as a PDF file from http://politicalwaffle.uk/journal/AJPS/article-full-text-pdf/86FDF2671482	  </self-uri>
	  
      <abstract><![CDATA[Hibiscus sabdariffa L. (Malvaceae) is a plant whose leaves and calyxes are integral to the local diet. The leaves are commonly used in sauce preparations, while the calyxes are utilized in making the local drink known as quot;bissap,quot; which is highly appreciated by the local population. Despite its socio-economic significance, the production of this species remains relatively low in Cocirc;te d#39;Ivoire. This decline in yield may be attributed to the lack of available information regarding the identification of different H. sabdariffa cultivars. To establish a comprehensive database, a survey and collection of various local cultivars of H. sabdariffa were conducted in 12 locations across Cocirc;te d#39;Ivoire. In total, 80 accessions were recorded, with 53.75% of them located in the North. Among these collected accessions, two primary cultivar types were identified based on calyx coloration: the red type (Hibiscus sabdariffa var sabdariffa) and the green type (Hibiscus sabdariffa var altissima). Within these two types, the presence of other morphotypes has been observed. Cultivation and trade of these cultivars are primarily carried out by women, with over 90% of them having no formal education. The price of calyxes of the red type varies from 3000 to 4700 FCFA per kg, providing an opportunity for farmers to diversify their sources of income. The various accessions collected thus constitute a substantial genetic reservoir necessary for the development of new H. sabdariffa varieties.

	 

	Key words: Hibiscus sabdariffa, ex-situ, Collection, Seed genebank, Cocirc;te d#39;Ivoire.]]></abstract>
    </article-meta>
  </front>
      <body/>
    <back>
		<ref-list>
			<title>References</title>
						<ref id="ref1">
				<label>1</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Abeda ZH, Sie RS, Ayolie K, Yapo SES, Coulibaly S, Kouassi KM, Kouakou TH (2015). Free Radical Scavenging Properties and Antioxidant Activities of Some Anthocyanins Purified from Roselle (Hibiscus sabdariffa L.) Callus Using In-Vitro Tests. Research Journal of Pharmaceutical, Biological and Chemical Sciences 6(6): 320-329.]]>
				</mixed-citation>
			</ref>
						<ref id="ref2">
				<label>2</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Adoukonou-Sagbadja H, Dansi A, Vodouhe R, Akpagana K (2006). Indigenous knowledge and traditional conservation of Fonio millet (Digitaria exilis Stapf, Digitaria iburua Stapf) in Togo. Biodiversity and Conservation 15:2379-2395.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref3">
				<label>3</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Alassi CA, Ewdj EBK, Adomou AC (2017). Diversit varitale et caractrisation agro-morphologique des varits locales de Hibiscus sabdariffa (bissap) au Sud et au Centre du Bnin: potentiel de valorisation: potentiel de valorisation. Bulletin de la Recherche Agronomique du Bnin (BRAB). Numro spcial cologie Applique, Flore  Faune (EAFF). pp. 46-65.]]>
				</mixed-citation>
			</ref>
						<ref id="ref4">
				<label>4</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Baudoin JP, Demol J, Louant BP, Marchal R, Guy M, Otoul E (2001). Amlioration des plantes : application aux principales espces cultives en rgions tropicales. Gembloux (Belgique): Presses Agronomiques de Gembloux, 583p.]]>
				</mixed-citation>
			</ref>
						<ref id="ref5">
				<label>5</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Boulanger J, Follin JC, Bourely J (1984). Les hibiscus textiles en Afrique tropicale, 1re partie: conditions particulires de production du kenaf et de la roselle. 5th Edition.]]>
				</mixed-citation>
			</ref>
						<ref id="ref6">
				<label>6</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ciss M, Dornier M, Sakho M, Diop C, Reynes M, Sock O (2008). La production de bissap (Hibiscus sabdariffa L.) au Sngal. Fruits 64(1):111-124.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref7">
				<label>7</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Dansi A, Adoukonou-Sagbadja H, Vodouhe R (2010). Diversity, conservation and related wild species of Fonio millet (Digitaria spp.) in the northwest of Benin. Genetic Resource and Crop Evolution 57:827-839.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref8">
				<label>8</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Diouf M, Diop M, Lo C, Drame Ka, Sene E, Ba Co, Gueye M, Faye B (1999). Prospection de lgumes feuilles traditionnels de type africain au Sngal. In Biodiversity of traditional leafy vegetables in Africa. Editors Chweya JA, Eyzaguire P. International Plant Genetic Resources Institute (IPGRI). Rome, Italie pp. 111-150.]]>
				</mixed-citation>
			</ref>
						<ref id="ref9">
				<label>9</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Diouf M, Gueye M, Faye B, Dieme O, Gningue D, Ba CO (2004). Rgnration et caractrisation daccessions de bissap, nib, amarante et nbday. Rapport de fin de projet  Gestion du germoplasme des lgumes feuilles traditionnels au Sngal  Projet IPGRI-ISRA. ISRA-CDH 84 p.]]>
				</mixed-citation>
			</ref>
						<ref id="ref10">
				<label>10</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Fall T (2001). Les fruits forestiers les plus importants au Sngal. In Le potentiel agroalimentaire du Sngal pour une meilleure scurit et sret alimentaire. Fte de la Science, Novembre 2013, Dakar, Institut Franais.]]>
				</mixed-citation>
			</ref>
						<ref id="ref11">
				<label>11</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Fane M, Barry A, Diwara M, Samak D, Babana A, Tounkara F, Diarra B (2021). Qualit nutritive du datou (Hibiscus sabdariffa) produit au mali: estimation de sa teneur en acide gras. Revue Malienne de Science et de Technologie 2(5):25-32.]]>
				</mixed-citation>
			</ref>
						<ref id="ref12">
				<label>12</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Faye A, Fall A, Tiffen M, Mortimore M, John N (2001). Bissap, Karad, Oseille de Guine; Aromates, pices et condiments du monde entier. Drylands Research Working paper 11 p.]]>
				</mixed-citation>
			</ref>
						<ref id="ref13">
				<label>13</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Garcia T, Duitama J, Zullo SS, Dohle S, Palkovic A, Skeen P, Bermudez-Santana CI, Debouck DG, Martnez-Castillo M, Gepts P, Chacn-Snchez MI (2021). Comprehensive genomic resources related to domestication and crop improvement traits in Lima bean. Nature Communications 12:702.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref14">
				<label>14</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ouangraoua JW, Kiebre M, Traore ET, Kiebre Z, Ouedraogo HM, Sawadogo M (2021). Caractrisation ethnobotanique de lOseille de Guine (Hibiscus Sabdariffa L.) de louest du Burkina Faso. International Journal of Innovation and Applied Studies 32(3):437-448.]]>
				</mixed-citation>
			</ref>
						<ref id="ref15">
				<label>15</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[R Core Team (2021). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL:
				
					View]]>
				</mixed-citation>
			</ref>
						<ref id="ref16">
				<label>16</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ross IA (2003). Hibiscus sabdariffa. In Medicinal Plants of the World, 2nd edn. Humana Press: New Jersey 1:267-275.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref17">
				<label>17</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Salah AM, Gathumbi J, Vierling W (2002). Inhibition of intestinal motility by methanolic extracts of Hibiscus sabdariffa L. (Malvaceae) in rats. Phytotherapy Research 16:283-285.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref18">
				<label>18</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Si RS, Akaffou DS, Kone D, Dogbo DO, Assohoun V, Charles G, Branchard M (2008). Influence des conditions de culture et du substrat sur le dveloppement des boutures de loseille de Guine : Hibiscus sabdariffa L. (Malvaceae). Agronomie Africaine 20(2):141-149.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref19">
				<label>19</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Si RS, Akaffou SD, Ska D, Konan KJL, Toueix KL, Charles G, Dj Y, Branchard M (2009). Caractrisation de la diversit et valuation agromorphologique dune collection dHibiscus sabdariffa L. en Cte dIvoire. Afrique Science 5(3):65-76.]]>
				</mixed-citation>
			</ref>
						<ref id="ref20">
				<label>20</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Si RS, Gilbert C, Hamidou FS, Toueix Y, Dj Y, Sangar A, Branchard M (2010). Protocols for callus and somatic embryo initiation for Hibiscus sabdariffa L. (Malvaceae): Influence of explant type, sugar, and plant growth regulators. Australian Journal of Crops Science 4(2):98-105.]]>
				</mixed-citation>
			</ref>
						<ref id="ref21">
				<label>21</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Sourabie S, Zerbo P, Yonli D, Joseph I, Boussim JI (2020). Connaissances traditionnelles des plantes locales utilises contre les bio-agresseurs des cultures et produits agricoles chez le peuple Turka au Burkina Faso. International Journal Biological and Chemical Sciences 14(4):1390-1404.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref22">
				<label>22</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Soro LC, Kon MB, Kouadio AKK, Atchibri ALO (2022). Influence de la cuisson  leau et du schage sur la valeur nutritionnelle de trois lgumes feuilles (Hibiscus sabdarifa, Solanum nigrum et Corchorus olitorius) consomms en Cte dIvoire. International Journal of Biological and Chemical Sciences 16(1):34-41.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref23">
				<label>23</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Wang CJ, Wang JM, Lin WL, Chu CY, Chou FP, Tseng TH (2000). Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats. Food and Chemical Toxicology 38(5):411-416.
					]]>
				</mixed-citation>
			</ref>
					</ref-list>
	</back>
    </article>