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  <front>    <journal-meta>
      <journal-title>African Journal of Plant Science</journal-title>
      <issn pub-type="epub">1996-0824</issn>      <publisher>
        <publisher-name>Academic Journals</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.5897/AJPS2024.2370</article-id>
      <title-group>
        <article-title><![CDATA[Allelopathy as a tool for invasiveness of Tithonia diversifolia extracts through in vitro suppression of crop seeds’ germination]]></article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author" xlink:type="simple">
        		        	<name name-style="western">
	            <surname>Musabayana</surname>
            <given-names>Zivanayi</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Mandumbu</surname>
            <given-names>Ronald</given-names>
	          </name>	
        		        	<name name-style="western">
	            <surname>Mapope</surname>
            <given-names>Nyamande</given-names>
	          </name>	
        	        </contrib>
      </contrib-group>
      <author-notes>
		<corresp id="cor1">* E-mail: <email xlink:type="simple">musabayana77@gmail.com</email></corresp>
      </author-notes>
      <pub-date pub-type="collection">
        <year>2024</year>
      </pub-date>
      <pub-date pub-type="epub">
      	<day>31</day>
        <month>07</month>
        <year>2024</year>
      </pub-date>
      <history>
      			<date date-type="received">
			<day>14</day>
			<month>05</month>
			<year>2024</year>
		</date>
						<date date-type="accepted">
			<day>14</day>
			<month>06</month>
			<year>2024</year>
		</date>
			  </history>
      <volume>18</volume>
      <issue>4</issue>
	  	  <fpage>28</fpage>
	  <lpage>40</lpage>
      <permissions>
		<license xlink:type="simple">
			<license-p>
			This is an open-access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
			</license-p>
		</license>
	  </permissions>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/AJPS/article-abstract/161F26F72386">
		This article is available from http://politicalwaffle.uk/journal/AJPS/article-abstract/161F26F72386	  </self-uri>
	  <self-uri xlink:href="http://politicalwaffle.uk/journal/AJPS/article-full-text-pdf/161F26F72386">
		The full text article is available as a PDF file from http://politicalwaffle.uk/journal/AJPS/article-full-text-pdf/161F26F72386	  </self-uri>
	  
      <abstract><![CDATA[Wild sunflower (Tithonia diversifolia) is an invasive weed in both tropical and subtropical cultivated lands and propagates through seeds and plant cuttings. Invasive plants have distinctive functional traits that enable their invasiveness. The objective of this study was to determine the in vitro suppression of germination of crop seeds by extracts of T. diversifolia. Two experiments were carried out, one in the laboratory and the other in the greenhouse. The laboratory experiment was set up in a completely randomized design, while the greenhouse experiment was set up in a Randomized Complete Block Design. The blocking factor for the greenhouse experiment was the distance from the greenhouse door. Both experiments were replicated five times. T. diversifolia parts (leaves, stem, roots, and whole plant) were used in this experiment. The Genstat statistical package was used to analyze the data. Results showed that whole plant, leaf, stem, and roots extracts significantly (plt;0.001) inhibited germination, radicle, plumule, stem girth extension, and chlorophyll content of maize, sunflower, soybeans, sorghum, rapoko grass, and cowpeas. This study confirmed that T. diversifolia parts suppressed germination, radicle, plumule, stem girth extension, and chlorophyll content of maize, sunflower, soybeans, rapoko grass, and cowpeas. Therefore, allelopathy contributes to the invasiveness of the weed.

	 

	Key words: Growth inhibition, invasive, traits, colonize.]]></abstract>
    </article-meta>
  </front>
      <body/>
    <back>
		<ref-list>
			<title>References</title>
						<ref id="ref1">
				<label>1</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Bano S, Ullah MA, Khaliq A, Abbasi KH, Khanum S (2012). Effects of aqueous extract of sun-dried neem (azadirachta indica) leaves on wheat and wheat weeds (wild oat and dumbi sitti) in Vitro. International Research Journal of Pant Science 3(4):69-73.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref2">
				<label>2</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Carboni M, Mnkemller T, Lavergne S, Choler P, Borgy B, Violle C, Essl, F, Roquet, C, Munoz F, DivGrass C, Thuiller W (2016). What it takes to invade grassland ecosystems: Traits, introduction history and filtering processes. Ecology letters 19(3): 219-229.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref3">
				<label>3</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Chukwuka KS, Obiakara MC, Ogunsumi IA (2014). Effects of aqueous plant extracts and inorganic fertilizer on the germination, growth and development of maize (Zea mays L.). Journal of Agricultural Sciences, Belgrade 59(3): 243-254.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref4">
				<label>4</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Gharde Y, Singh PK, Dubey RP, Gupta, PK (2018). Assessment of yield and economic losses in agriculture due to weeds in India. Crop Protection 107:12-18.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref5">
				<label>5</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Muoghalu JI, Chuba DK (2005). Seed germination and reproductive strategies of Tithonia diversifolia (Hemsl.) Gray and Tithonia rotundifolia (PM) Blake. Applied Ecology and Environmental research 3(1):39-46.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref6">
				<label>6</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Musyimi DM, Kahihu SW, Buyela DK, Sikuku PA (2012). Allelopathic effects of Mexican sunflower [Tithonia diversifolia (Hemsl) A. Gray] on germination and growth of Spiderplant.]]>
				</mixed-citation>
			</ref>
						<ref id="ref7">
				<label>7</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Olakojo SA (2004). Evaluation of some maize inbred lines for tolerance to Striga lutea (Lour) in Southern Guinea savanna ecology. Journal of Food Agriculture and Environment 2:256-259.]]>
				</mixed-citation>
			</ref>
						<ref id="ref8">
				<label>8</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Otusanya O, Ilori O (2014). Studies on the allelopathic effects of Tithonia rotundifolia on the germination and seedling growth of some legumes and cereals. International Journal of Biology 6(1):38-47.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref9">
				<label>9</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Perkins LB, Nowak RS (2013). Invasion syndromes: hypotheses on relationships among invasive species attributes and characteristics of invaded sites. Journal of Arid Land 5:275-283.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref10">
				<label>10</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Pyek P, Richardson DM (2007). Traits associated with invasiveness in alien plants: where do we stand? Biological Invasions pp. 97-125.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref11">
				<label>11</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Rejmnek M, Huntley BJ, Le Roux JJ, Richardson DM (2017). A rapid survey of the invasive plant species in western Angola. African Journal of Ecology 55(1):56-69.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref12">
				<label>12</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Rejmnek M, Richardson DM (2013). Trees and shrubs as invasive alien species-2013 update of the global database. Diversity and distributions 19(8):1093-1094.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref13">
				<label>13</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Rodenburg J, Demont M, Zwart SJ, Bastiaans L (2016). Parasitic weed incidence and related economic losses in rice in Africa. Agriculture, Ecosystems  Environment 235:306-317.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref14">
				<label>14</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Ronald M, Peter J, Charles K, Tibugari H (2011). Integrated weed management in Zimbabwes smallholder sector, where are we? a review. Modern Applied Science 5(5):111.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref15">
				<label>15</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Rugare JT (2018). Allelopathic effects of green manure cover crops on the germination and growth of blackjack (Bidens pilosa L.) and rapoko grass [Eleusine indica (L.) Gaertn] (Doctoral dissertation, Stellenbosch: Stellenbosch University).]]>
				</mixed-citation>
			</ref>
						<ref id="ref16">
				<label>16</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Shackleton RT, Nunda W, Beale T, Van Wilgen BW, Witt AB (2019). Distribution of invasive alien Tithonia (Asteraceae) species in eastern and southern Africa and the socioecological impacts of T. diversifolia in Zambia. Bothalia-African Biodiversity  Conservation 49(1):1-11.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref17">
				<label>17</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Valk O, Dek, B, Trk P, Kelemen A, Miglcz T, Tth K, Tthmrsz B (2016). Abandonment of croplands: problem or chance for grassland restoration? Case studies from Hungary. Ecosystem Health and Sustainability 2(2):e01208.
					]]>
				</mixed-citation>
			</ref>
						<ref id="ref18">
				<label>18</label>
				<mixed-citation publication-type="other" xlink:type="simple">
				<![CDATA[Yaduraju NT, Rao AN (2013). Implications of weeds and weed management on food security and safety in the Asia-Pacific region. pp. 13-30.]]>
				</mixed-citation>
			</ref>
					</ref-list>
	</back>
    </article>